In vitro study of effective factors for the inhibitory assay on pancreatic lipase

Background: Pancreatic lipase is one of the safest targets anti-obesity drugs. To date, orlistat only pancreatic inhibitor approved for long-term treatment obesity. Therefore, there an elevated need to find new drugs this disease. Determining factors affecting test evaluate inhibitory activity in order build a standard assay procedure necessary. This will make it much easier researchers novel compounds that inhibit enzyme. Materials and method: The current study investigated influencing evaluated enzyme inhibition by spectrophotometric method at 405 nm using p-nitrophenyl palmitate as substrate. Results: With optimized conditions, gave results similar those published other authors. Conclusion: methodology work should be applied studies looking effective treat Key words: obesity, orlistat.